【摘要】 以籼粳交“密阳23/吉冷1号”的F2:3 代200个家系为作图群体,构建分子连锁图谱,并进行了F3 代家系的生长早期耐冷性鉴定和QTL分析。结果表明,幼苗期耐冷性、分蘖期耐冷性和低温下幼苗生长能力等生长早期耐冷性在F3 代表现为近似正态的连续分布,是由多基因控制的数量性状。在第1、5、9 染色体上分别检测到与幼苗期耐冷性相关的QTL各1个,其中qCTS1 对表型变异的解释率最大,达15.5%;在第2、3、7、9、11 染色体上分别检测到与分蘖期耐冷性相关的QTL各1个,而每个QTL对表型变异的解释率均较低;在第1、2、11、12染色体上分别检测到与低温下幼苗生长能力相关的QTL各1个,其中qGAS2 和qGAS12 对表型变异的解释率分别为26.6%和42.9%,是主效基因。更多还原

【Abstract】 The quantitative trait loci (QTL) for cold tolerance during early growth period in rice were identified by using F 2:3 population including 200 individuals and lines derived from a cross of indica and japonica rice ‘Milyang 23/Jileng 1’ with microsatellite markers.The evaluations of cold tolerance at the seedling stage, cold tolerance at the tillering stage, and the growth ability of seedling under low temperature were conducted. All traits above associated with cold tolerance at early gowth stage appeared a continuous distribution near to normal in F 3 lines, these traits were quantitative traits controlled by multi genes. Three QTL on chromosomes 1, 5 and 9, which associated with cold tolerance at the seedling stage were detected, among them, qCTS1 accounted 15.5% of observed phenotypic variation; Five QTL on chromosomes 2, 3, 7, 9 and 11, which associated with cold tolerance at the tillering stage were found, all of these QTL explained low ratio of observed phenotypic variation; Four QTL on chromosomes 1, 2, 11 and 12, which associated with the growth ability of seedling under low temperature were detected, among them, qGAS2 and qGAS12 explained 26.6% and 42.9% of observed phenotypic variation, respectively, suggesting they were major genes.更多还原