【摘要】 本文以染色体牙釉蛋白基因(AML)对牛肌肉基因组进行性别鉴定,为应用此基因进行胚胎鉴定提供理论依据。本研究应用奶牛染色体牙釉蛋白基因的DNA序列,设计了牛染色体特异性引物P1和P2,并通过聚合酶链反应(PCR)扩增牛肌肉组织中的X和Y染色体上的牙釉蛋白基因内第五内含子。最后依据其分子量的差异对电泳后的性染色体所呈现的不同区带进行区分,从而达到性别鉴定的目的,具有很高的应用价值。更多还原

【Abstract】 This paper using the gene of amelogenin detect dairy cow muscle genome to identify its sex. Basing on the amelogenin DNA sequence, we design a pair of special primer P1 and P2 a,nd amplify the fifth intron of amelogenin in cow muscle tissue X and Y chromosome. According to its difference, the author distinguish its electrophonic bands to detect its sex. This research has highly applicative value.更多还原