【摘要】 目的:探讨体外反搏对高胆固醇血症猪血管内皮细胞的保护作用及机制。方法:选雄性乳猪12头用高脂喂养建立动脉粥样硬化模型,对模型猪进行32h体外反搏治疗。用cDNA基因芯片对猪的动脉血管内皮细胞基因表达谱进行量化分析。结果:腹主动脉苏丹Ⅲ染色图像定量分析,高脂组:7.84%±2.37%;高脂加反搏组:1.26%±0.36%。两组差异有显著性意义(P<0.05)。冠状动脉左旋支内膜1:500扫描电镜显示:高脂+反搏组内膜的血管内皮细胞排列有序,细胞呈梭型排列与血流方向一致,表面光滑;高脂组血管内膜细胞排列紊乱,表面不光滑,有大量脱落细胞。显著改变的基因:芯片1(反搏组Cy3/高脂组Cy5):锌指蛋白0.33、MAP4K30.13、长链脂肪酸合成酶0.35、神经介素B受体0.19、GPX-13.26、整合素β10.06、CTGF0.28、eNOS3.1、VCAM-10.50;芯片2(高脂组Cy3/正常组Cy5):锌指蛋白23.06、MAP4K36.75、长链脂肪酸合成酶2.71、神经介素B受体6.68、GPX-10.63、整合素β16.29、CTGF2.45。结论:长期体外反搏可以改善内皮形态与功能,增加内皮细胞抗氧化作用,下调内皮细胞机械性刺激感应蛋白基因的表达,降低血管內皮细胞对LDL的摄取,及下调炎症因子相关基因的表达,起到抗动脉粥样硬化的作用。更多还原

【Abstract】 Objective: To evaluate the efficacy of external counterpulsation for prevention of atherosclerosis in hypercholesterolemia pig.Method:12 male infant pigs were fed with hypercholesterol diet to induce atherosclerosis. These model pigs were received ECP for 32h. The gene expression levels of ECP on endothelial cells were analysed by using cDNA microarray hybridization.Result:The ratios of sudan III area in celiac arota were 1.26%±0.36% and 7.84%±2.37% in ECP group and hypercholesterolemia group, respectively(P<0.05). Scanning electron microscope showed that the endothelial cells in coronary artery were aligned in the direction of blood flow in ECP group and disordered in hypercholesterolemia group. The significant changes of gene expression in microarray 1:zinc finger protein 0.13, MAP4K3 0.33, long-chain fatty acid coenzyme A ligase5 0.35, neuromedin B receptor 0.19, integrinsβ1 0.06, CTGF 0.28, GPX-1 3.26,eNOS 3.10, VCAM-1 0.50; in microarry 2:zinc finger protein 23.06, MAP4K3 6.75 long-chain fatty acid coenzyme A ligase5 2.71, neuromedin B receptor 6.68, integrinsβ1 6.29, CTGF 2.45, GPX-1 0.63.Conclusion: 32h ECP may reverse endothelial dysfunction in hypercholesterolemia pigs, down-regulate the gene expression of mechanosensing protein in endothelial cell membrane, reduce LDL uptake and inflammatory factors and increase antioxidants in endothelial cells. The changes of gene expression in endothelial cells suggested that ECP may reduce the trend of atherosclerosis.更多还原