【摘要】 从丝裂原刺激的鸭脾淋巴细胞中提取基因组 RNA,采用 RT-PCR 扩增鸭干扰素γ基因并进行了序列分析。序列分析结果表明,鸭干扰素γ基因序列全长477bp,开放阅读框架内401个核苷酸,共编码132个氨基酸。将鸭γ-IFN 基因定向克隆到原核表达载体pBV220中,重组质粒经酶切鉴定后,转化到宿主菌 DH5a 中,温度诱导表达,SDS-PAGE 分析,表达产物与标记抗鸡干扰素抗体出现 Dot-ELISA 阳性反应。更多还原

【Abstract】 The gene eneodingγ-interferon(IFN)of the duck was amplified using RT-PCR.Sequencing results demon- strated that total of the gene contain 477bp with a 401bp open reading fragment(ORF),encoding one 132aa protein.The expression plasmid,duck pBV220-IFN-γ,was transformed into DH5a Escherichia coli cell and induced with temperature. SDS-PAGE analysis and Dot-ELISA confirmed the expressed protein has biological function.更多还原