【摘要】 目的研究全反式维甲酸(all-trans-retinoicacid,at-RA)在单侧输尿管梗阻(unilateraluretrealobstructive,UUO)大鼠模型中对肾间质肌成纤维细胞(MyoF)的积聚及胶原Ⅲ(colⅢ)沉积的影响。方法将40只大鼠随机分为假手术组、UUO组、维甲酸大剂量组,维甲酸小剂量组,苯那普利组。后3组分别给予全反式维甲酸20mg、10mg·kg-1·d-1和苯那普利10mg·kg-1·d-1。术后第14天处死各组大鼠,用免疫组化方法测定α平滑肌肌动蛋白(αSMA)及colⅢ的表达。行HE、MASSON染色观察肾脏病理变化。结果全反式维甲酸可显著减少肾间质区MyoF积聚,减轻colⅢ的沉积,并改善肾脏的病理学变化。结论全反式维甲酸可通过减少肾间质区MyoF积聚,减轻colⅢ的沉积而改善UUO所致的肾间质纤维化。更多还原

【Abstract】 Objective To study the effect of all-transretinoic acid(at-RA) on theprocess of renal interstitial fibrosis following unilateral ureteral obstruction (UUO) inrats. Methods Fouty SD rats were randomly assigned into 5 groups :Two atRA treatment groups(10mg and 20mg·kg-1·d-1 respectively), benazepril treatment group(10mg·kg-1·d-1),UUOcontrol group and normal control group. All of rats were killed 14 days after surgery.Immunohistochemistry was performed on renal tissue to detect smooth muscle actin(α-SMA)which was a cytoskeletal marker of myofibroblast (MyoF) and collagen Ⅲ(col Ⅲ). Histologi-cal studies were also done by he and MASSON stainting. Results Fourteen days after surgery,interstitial myof infiltration and interstitial col Ⅲ deposition were all significantlyreduced by at-RA treatment. At-RA treatment also improved the histological changes of UUOrats. Conclusion Reduction of interstitial MyoF infiltration may be an important event bywhich at-RA prevents renal injury caused by UUO.更多还原