【摘要】 以一株降解氯氰菊酯的红球菌(Rhodococcussp.)CDT3为材料,研究了CDT3降解氯氰菊酯的最适条件(温度、pH值、接种量、培养时间、添加营养物).结果表明,在温度30℃,pH8.0时,CDT3对氯氰菊酯的降解率最高,添加少量葡萄糖、蛋白胨、酵母汁均能促进它对氯氰菊酯的降解.研究了CDT3降解氯氰菊酯的代谢途径,通过GC-MS分析,证实氯氰菊酯被CDT3降解后的产物是3-苯氧基苯甲酸(3-PBA)和二氯菊酸(DCVA),推测氯氰菊酯是由CDT3产生的羧酸酯酶降解的,作为验证,提取了CDT3的粗酶液,并且通过聚丙烯酰胺凝胶电泳(PAGE)检测了羧酸酯酶.更多还原

【Abstract】 Using a strain of cypermethrin-degrading Rhodococcus sp. CDT3 as material, the optimal conditions (temperature, pH value, inoculating dose, cultural time and added nutrition) for CDT3 to degrade cypermethrin was studied. In a shaky flask containing 100mg/L cypermethrin, the degrading rate of CDT3 on cypermethrin was highest at temperature 30℃, pH8.0; adding little amounts of glucose, tryptone, yeast extract could promote its degradation of cypermethrin. The metabolic pathway of cypermethrin degraded by CDT3 was studied; through GC-MS analysis, The degraded products of cypermethrin were 3-Phenoxybenzoic acid (3-PBA) and DCVA [cis, trans-3- (2,3-dichloroethenyl)-2,2-Dimethylcyclopropane- carboxylate]. It was suggested that cypermethrin came from the degrading of carboxylesterase produced from CDT3; for confirming this the crude enzyme liquor of CDT3 was extracted and carboxylesterase was detected through polyacrylanide gel electrophoresis (PAGE).更多还原