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一株溶藻细菌的溶藻特性及其鉴定

Algae-lytic character and its identification of one Algae-Lysing bacterium.

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【作者】 裴海燕胡文容曲音波母锐敏李小彩

【Author】 PEI Hai-yan1, HU Wen-rong2*, QU Yin-bo1, MU Rui-min2, LI Xiao-cai2 (1.State Key Laboratory of Microbial Technique, Shandong University, Jinan 250100, China;2.School of Environment Science and Engineering, Shandong University, Jinan 250061, China). China Environmental Science, 2005,25(3):283~287

【机构】 山东大学微生物技术国家重点实验室山东大学环境科学与工程学院山东大学环境科学与工程学院 山东济南250100山东济南250061山东济南250100山东济南250061

【摘要】 从海绵固定化微生物系统中分离获得1株溶藻细菌P15,对其溶解铜绿微囊藻(Microcystisaeruginosa)、栅藻(Scenedesmus)及小球藻(Chlorella)的效果、溶藻方式进行了研究,并利用16SrDNA序列分析法进行了鉴定.结果表明,初始菌浓度大于2.4×107个/mL时,P15菌具有理想的溶藻效果,48h后铜绿微囊藻去除率可达到65.84%,栅藻及小球藻也能很好地去除.P15菌革兰氏染色阳性,可运动;能利用乳酸胺等32种常见碳源.P15菌株与多株红球菌的16SrDNA核苷酸序列的同源性均在99.7%以上,归属于红球菌属.

【Abstract】 One algae-lysing bacterium P15 was isolated from microbiosystem immobilized with sponge. Its lytic effects on Microcystis aeruginosa, Scenedesmus and Chlorella and the algae lysing mode were studied and identified utilizing 16S rDNA sequence analysis technique. When the initial densitiy exceeded 2.4×107 cells/mL, the strain P15 possessed ideal lytic effect and the removal rate of Microcystis aeruginosa would reach 65.84% after 48 hours, and also removed very well the Scenedesmus and Chlorella. The P15 bacterium was gram-positive and mobile; and could utilize 32 kinds of common carbon sources including Lactamide, etc. The sequence analysis of 16S rDNA showed that the 16S rDNA nucleotide sequence homology of P15 strain with many strains of Rhodococcus all reached above 99.7%, P15 belonging to Rhodococcus.

【基金】 中日合作项目(003150103);高等学校博士学科点专项科研基金资助项目(20020422045);山东省自然科学基金资助项目(Z2003B01)
  • 【文献出处】 中国环境科学 ,China Environmental Science , 编辑部邮箱 ,2005年03期
  • 【分类号】X173
  • 【被引频次】50
  • 【下载频次】433
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