【摘要】 目的研究果糖诱导高血压和胰岛素抵抗大鼠血管内皮素-1(ET-1)及内皮素-A受体(ETA-R)和内皮源性一氧化氮合酶(eNOS)的变化,探讨内皮功能变化在果糖诱导高血压和胰岛素抵抗中的作用。方法雄性Sprague-Dawley(SD)大鼠饮用10%果糖水3周,尾套法测量血压,并留取血清及尿标本分别测定血清胰岛素、血脂、血糖及血和尿中血ET-1水平后,处死大鼠。ELISA法测定血清及尿中ET-1水平,RT-PCR法测定大鼠主动脉中ET-1及ETA-R表达,Western杂交检测eNOS的表达情况。结果与正常饮水大鼠相比,高果糖饮水3周后,大鼠血压明显升高[(132.8±1.49 vs 120.5±1.7)mmHg,P<0.01]、血胰岛素水平亦明显高于正常饮水组[(12.2±2.3 vs 9.5±1.2)mIu/L,P<0.05]。两组间血清ET-1的水平并没有显著差异,但尿中ET-1的水平,果糖饮水组显著高于正常对照组。果糖饮水组大鼠主动脉ET-1和ETA-R的mRNA水平明显升高,而eNOS蛋白质水平显著低于正常组。结论ET-1和ETA-R高表达及eNOS表达的降低参与了果糖诱导高血压和高胰岛素血症的形成。更多还原

【Abstract】 Objective To examine the vascular of endothelin-1(ET-1),endothelin-A receptor(ETA-R) and endithelial nitric oxide synthase(eNOS) in fructose-induced hypertension and hyperinsulinemia rats. Methods Male Sprague-Dawley rats fed on high-fructose(10%) water developed mild hypertensive models within 3 weeks. Blood glucose,insulin and ET-1 were determined. Vascular ET-1 and ETA-R gene expressions were measured by RT-PCR. The expression of vascular eNOS was measured by Western blot. ResultsFructose-fed rats developed systolic hypertension(132.8±1.5 vs 120.5±1.7 mm Hg,P<0.01) and hyperinsulinemia(12.2±2.3 vs 9.5±1.2 mIu/L,P<0.05) after 3 weeks. Although plasma ET-1 levels did not differ between the rat groups,urine ET-1 levels were significantly higher in fructose fed rats than in controls. Fructose-fed rats had higher vascular ET-1 and ETA-R gene expression than that in control rats,associated with lower level of eNOS was also shown. Conclusion These findings suggest that elevated vascular expression of ET-1 and ETA-R and the lower eNOS genes may mediate the development of hypertension and hyperinsulinemia in rats fed a fructose-rich diet.更多还原