【摘要】 目的:了解不孕妇女生殖道解脲支原体(ureaplasmaurealyticum,UU)、生殖支原体(myoplasmagenitalium,MG)、穿通支原体(myoplasmapenetrans,MPe)、发酵支原体(myoplasmafermentans,MF)以及沙眼衣原体(chlamyditrachomatis,CT)的感染情况并探讨其与不孕症之间的关系。方法:收集不孕妇女之宫颈分泌物,应用荧光定量PCR(FQ-PCR)及套式PCR(nPCR)技术进行检测。结果:在80例不孕妇女宫颈液中,UU、MG、MPe、MF、CT的检出率分别为55.00%、6.25%、3.75%、1.33%、7.50%;原发和继发性不孕患者之间UU、MG、MPe、MF、CT的检出率无显著性差异(P>0.05)。结论:不孕妇女生殖道中支原体感染率较高,以UU为主,检出穿通支原体、发酵支原体两种新近发现的致病支原体,提示UU、MG、MPe、MF和CT感染均和不孕症相关。更多还原

【Abstract】 Objective:To study the infections of ureaplasma urealyticum(Uu), mycoplasma genitalium(Mg),mycoplasma penetrans(Mpe) ,mycoplasma fermentans(Mf)and chlamydi trachomatis(CT) in infertile woman.Methods:The secretions from cervical canal were collected..Fluorescence quantitative PCR(FQ-PCR) and nest PCR(nPCR) were performed to detect Uu, Mg, Mf, Mpe and CT.Results:Uu (55.00%), Mg(6.25%), Mpe(3.75%),Mf(1.33%) and CT(7.50%) were isolated from 80 infertility cases;The difference of positive ratio of Uu,Mg,Mpe,Mf ,CT between primary and secondary infertility were not insignificant(P>0.05).Conclusion:The infection rate of mycoplasmas especially the Uu were more higher ; and new type pathogenic mycoplasmas (Mpe,Mf) were detected.更多还原