【摘要】 目的　建立延边州结核分枝杆菌IS6 1 1 0RFLP指纹图谱 ,并分析朝鲜、汉两族的图谱特征。方法　 97株结核分枝杆菌分离株 ,提取DNA ,经酶切 ,电泳 ,Southem转印 ,探针杂交 ,发光自显影技术得到IS6 1 1 0RFLP图谱 ;按其指纹图谱特征的同源性高低进行分析。结果　延边州结核分枝杆菌IS6 1 1 0拷贝数目平均为 1 3个 ;5 7.7%菌株可成簇 ,具有“北京基因型”特征 ;IS6 1 1 0RFLP图谱特征分布无地域差异 ,无民族差异。结论　延边州结核分枝杆菌分离株同北京分离株有较近的亲缘关系 ,但其多态性较高。更多还原

【Abstract】 Objective To establish IS6110 RFLP fingerprint patterns of Mycobacterium tuberculosis isolated from Yanji City and analyze the national characteristics of Han and Korean nationalities.Methods To abstract the DNA from 97 mycobacterium tuberculosis strains,using endonuclease,southern blot,probe hybridization and chemiluminescence to get IS6110 RFLP fingerprint patterns,analyze the characteristics according to their homology of IS6110 RFLP fingerprint.Results The characteristics of IS6110 RFLP patterns of mycobacterium tuberculosis strains isolated from Yanji City showed:(1)The average of the copies of IS6110 is 13.(2)The clustering rate of strains was 57.7%,they had the characteristics of “Beijing genotype strains".(3)The characteristics of IS6110 RFLP fingerprint patterns were not related to the geographic and ethnic distribution of the patterns.Conclusion The isolated strains of Mycobacterium tuberculosis from Yanji City was closely relative to “Beijing genotype strains”,but there was a feature specially,that was the high polymorphism.更多还原