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云南常见药用红景天的RAPD分析

RAPD analysis of common medicinal plants of Rhodiola L. in Yunnan Province

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【作者】 虞泓朱荣勋李永谊和云峰

【Author】 YU Hong~(1,2), ZHU Rong-xun~(1), LI Yong-yi~(1), HE Yun-feng~(1) (1. Yunnan Inmol Laboratory of Biotechnology, Kunming 650106, China; 2. Laboratory of Ecological Genetics, College of Life Science, Yunnan University, Kunming 650091, China)

【机构】 云南英茂生物技术实验室云南英茂生物技术实验室 云南昆明65010云南大学生命科学学院生态遗传学实验室云南昆明650091云南昆明650106云南昆明650106

【摘要】 目的研究云南常见药用植物红景天的遗传背景。方法采用RAPD技术对3种6个居群59个红景天样品进行了遗传关系研究。结果大花红景天3个居群的多态位点百分率(PPB)分别为37.97%、46.15%、39.45%,Nei’s基因多样性(h)分别为0.1103、0.1437、0.1371,Shannon’s信息指数(I)分别为0.1736、0.2221、0.2067,物种水平的PPB为70.47%,I为0.2583,基因分化系数Gst为0.2630;长鞭红景天2个居群的PPB分别为43.67%、44.42%,I分别为0.2153、0.2174,物种水平的PPB为65.51%,Gst为0.3132,I为0.3139;云南红景天的PPB为36.97%,I为0.1881。结论RAPD分子标记可很好地用于红景天物种的分子鉴定和遗传背景研究。

【Abstract】 Objective To analyze the hereditary background of common species from Rhodiola L. in Yunnan Province. Methods Fifty-nine samples of six populations in three natural medicinal species from Rhodiola L. were studied by RAPD method. Results The percentage of polymorphic bands (PPB) of three populations from R. crenulata were 37.97%, 46.15%, and 39.45% respectively; the Nei’s gene diversities (h) were 0.110 3, 0.143 7, and 0.137 1, respectively; the Shannon’s information indes (I) were 0.173 6, 0.222 1, and 0.206 7, respectively; at the special level, the PPB was 70.47%, I was (0.258 3); the genetic differentiation (Gst) was 0.263 0. The PPB of two populations from R. fastigiata were 43.67% and 44.42%, I were 0.215 3 and 0.217 4, respectively; at the special level, the PPB was 65.51%, Gst and I were 0.313 2 and 0.313 9, respectively; the PPB of R. yunnanensis was 36.97%, I was 0.188 1. Conclusion RAPD molecular marker could be used to molecular authentication and genetic diversity analysis of medicinal species from Rhodiola L.

【基金】 中药现代化科技产业(云南)基地专项(2002ZY-18)
  • 【文献出处】 中草药 ,Chinese Traditional and Herbal Drugs , 编辑部邮箱 ,2005年01期
  • 【分类号】R284.1
  • 【被引频次】42
  • 【下载频次】315
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