【摘要】 目的优选白芨多糖硫酸酯化的最佳工艺。方法采用氯磺酸-吡啶法合成白芨多糖硫酸酯。以取代度和产物量为指标,采用L9(34)正交设计对试剂比例、反应温度和反应时间进行优选。结果吡啶和氯磺酸比例6∶1,反应温度85℃,反应时间2h为比较适宜的工艺条件。结论白芨多糖硫酸酯化优化的工艺条件方法可行,为进一步扩大试验提供了有价值的参数。更多还原

【Abstract】 Objective To optimize the sulfation-conditions of Bletilla striata polysaccharide （BSPS）. Methods BSPS was sulfated with chlorsulfonic acid-pyridine. Reagent proportion, reaction temperature, and reaction time were selected with substitution degree and yield as index by L₉（3⁴） orthogonal design. Results Results showed that the condition with 6∶1 volume ratio of pyridine and chlorine sulfonic acid, reaction temperature of 85 ℃, reaction time of 2 h was the optimum. Conclusion The optimal method for sulfation of BSPS is feasible, and it provides valuable parameters for further enlarged test.更多还原