【摘要】 目的:GC内标法测定猴头多糖中脂肪酸的含量。方法:猴头多糖中脂肪酸种类由GCMS法测定。含量由GC内标法确定:取猴头多糖样品1.5g,加入含内标0.5mg的硫酸甲醇溶液(1∶20)5mL,80℃甲酯化3h,环己烷萃取4次,定容,GC分析。色谱柱为DB1,初始温度60℃,程序升温10℃·min-1至200℃,保持30min。检测器(FID)250℃,进样口250℃,载气为高纯氮气。结果:脂肪酸甲酯得到很好的分离,在0.4～3.6mg·mL-1的浓度范围内呈线性,稳定性和回收率良好。结论:该方法准确、重现性好,适合于测量猴头多糖中脂肪酸的含量。更多还原

【Abstract】 AIM: To assay fatty acids in Hericium Erinaceus polysaccharide by GC for establishing a method for quantitative determination of fatty acids. METHODS: The kinds of fatty acids were detected by GC-MS. The fatty acids contents were determined by GC: The sample was dissolved in sulphuric acid-methanol (1∶20) containing 0.5 mg heptadecanoic acid methyl ester and kept at 80°C for 3 hr. Then, the solution was extracted by hexamethylene four times and diluted to 1 mL. The extraction (0.4 μL) was injected on a DB-1 column, using nitrogen as the carrier gas. The temperature was raised from 60°C to 200°C at the rate of 10°C·min -1 , and subsequently sustained for 30 min. The injection port and detector temperature were both at 250°C. RESULTS: The fatty acids were separated completely. The linear ranges were 0.4-3.6 mg·mL -1 . RSD<3% and the recoveries >97%. CONCLUSION: The fatty acids can be determined accurately by this method.更多还原