【摘要】 目的:研究白条党参的指纹图谱。方法:高效液相色谱法,二级管阵列检测器,甲醇水梯度流动相,流速0.8mL·min-1;测定指标成分苍术内酯Ⅲ的含量,用蒸发光散射检测器(ELSD)和二级管阵列检测器(PAD),流动相:甲醇水(67∶33),流速1.0mL·min-1,柱温30°C,蒸发光散射检测器,载气流速2.2L·min-1;漂移管温度80°C。PAD检测波长220nm。结果:分析了25个共有峰,平均相似度为98.12eNoR。用指标成分苍术内酯Ⅲ进行了定位。结论:白条党参样品指纹图谱可作为白条党参药材极性部分的特征图谱。更多还原

【Abstract】 AIM To establish chromatography fingerprint of Radix Codonopsis. METHODS HPLC was applyed on a Hypersil ODS column with water-methanol gradient elution, flow-rate of 0.8 ml·min -1 . Determination of content of atractylenodie Ⅲ adopted ELSD and PAD with methanol-water(67∶ 33), flow-rate of 1.0 ml·min -1 , column temperature at 30°C , ELSD:carried-gas flow-rate of 2.2 L·min -1 , drift-tuber temperature was 80°C ;PAD:wavelength was 220 nm. RESULTS 25 common peaks were picked up, their similarity among 10 batchs of samples was more than 98.12 % based on marker composition(Atractylenolide Ⅲ). CONCLUSION The chromatography fingerprint of Baitiao Radix Codonopsis could be used as their characteristic chromatography fingerprint of hydrophobic fraction.更多还原