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苯酚硫酸法测定杂多糖含量的研究
Determination of sugars in heteropolysaccharide by phenol-sulfuric acid method
【摘要】 目的:确立一种能够准确测定杂多糖中总糖含量的实验方法。方法:采用酸水解多糖,GC法分析各组成糖比例,配制相应的标准溶液,用苯酚硫酸法测定多糖含量。结果:实验结果良好,标准糖混合溶液在8.56~38.52mg·L-1浓度
【Abstract】 AIM: To develop a new method based on phenol-sulfuric reaction for the determination of hetero-polysaccharide. METHODS: The proportion of sugars in the hetero-polysaccharide was determined by GC. According to standard solution obtained, Heteropolysaccharide can be determined through the standard curve. ( RESULTS:)The linear relationship of standard solution of mixed sugars was at the range of 8.56-38.52 mg/L-1. CONCLUSION: GC and phenol-sulfuric acid method can be used to determine the heteropolysaccharide accurately.
【基金】 国家重点科技项目(专题合同编号:969010668)
- 【文献出处】 中成药 ,Chinese Traditional Patent Medicine , 编辑部邮箱 ,2005年06期
- 【分类号】O636.1
- 【被引频次】236
- 【下载频次】5049