【摘要】 目的:测定苦碟子注射液(抱茎苦卖菜 )中腺嘌呤核苷的含量。方法:采用高效液相色谱法测定,色谱柱为Shim packCLC ODS柱(6. 0mm×150mm, 5μm),流动相为甲醇 乙腈 水 ( 3∶4∶93 ),流速 1mL·min-1,检测波长为 260nm。结果:苦碟子注射液中腺嘌呤核苷含量为 0. 15mg·10mL-1。加样回收率为 98. 4 (n=5)。结论:高效液相色谱法可用于评价苦碟子注射液的质量。更多还原

【Abstract】 AIM: To determine adenosine content in Kudiezi Injection(Ixeris sonchifolia Hance). METHODS: HPLC was used with Shim-pack CLC-ODS(6.0 mm×150 mm,5 μm). The mobile phase consisted of methanol-acelonitrile-0.025 mol·L -1 phosphoric acid (3∶4∶93). The flow rate was 1 mL·min -1 . The UV detection wavelength was at 260 nm. RESULTS: The adenosin content in Kudiezi Injection was 0.15/10 mL. The average recovery was 98.4%(n=5). CONCLUSION: HPLC can be used for the quality control of Kudiezi Injection Injection.更多还原