【摘要】 目的:建立复方首乌颗粒(何首乌、马尾莲、冬凌草等)的质量标准。方法:采用TLC法对处方中何首乌、马尾莲、冬凌草进行鉴别;用HPLC法测定 2, 3, 5, 4’ 四羟基二苯乙烯 2 0 β D 葡萄糖苷的含量。结果:定性鉴别分离度好,专属性强; 2, 3, 5, 4’ 四羟基二苯乙烯 2 0 β D 葡萄糖苷的含量测定线性范围为 0. 04~0. 72μg(r=0. 999 9,n=6);平均回收率为 99. 44 (RSD=1. 3 ,n=6);结论:所建立的方法简便、准确可靠,重复性好,可作为复方首乌颗粒的质量控制标准。更多还原

【Abstract】 AIM:To establish the quality standard of Compound Shouwu Granule (Radix Polygoni multiflori,Radix et Rhizoma Thalictri Baicalensis,Herba Rabdosiae Rubescentis,etc.) METHODS:TLC was performed to identify Radix Polygoni Multiflori,Radix et Rhizoma Thalictri Baicalensis,Herba Rabdosiae Rubescentis.HPLC was used to determine 2,3,5,4’-tetrahydroxystilbene-2-0-β-D-glucoside. RESULTS:The study on the quality control showed that the characteristic of identification by TLC was distinct and highly specific.The quantitative evaluation had the linear range of 0.04-0.72 μg.The average recovery was 99.99% and RSD was 1.3%. CONCLUSION: The method for identification and quantitation was simple,accurate,realizable and reproducible.It can be used effectively for the quality control of Compound Shouwu Granule.更多还原