【摘要】 目的:通过观察内毒素休克大鼠脑皮质中NOS活性、NO含量和TLR4 MRNA的表达及人参二醇组皂苷(PDS)对其的影响,探讨内毒素引起脑组织损伤的分子机制。方法:大鼠随机分为实验对照(CONTROL)组、内毒素休克(LPS)组、地塞米松(LPS+DEX)组和人参二醇组皂苷(LPS+PDS)组。大鼠静脉注射内毒素(4 MG/KG)4H后测定脑组织中NOS活性、NO含量及TLR4 MRNA的表达。结果:LPS+DEX组和LPS+PDS组NOS活性、NO2-/NO3-含量显著低于LPS组(P<0．05),TLR4 MRNA表达亦明显低于LPS组。结论:PDS能够下调脑组织中TLR4 MRNA的表达,降低 NOS活性、NO含量,对中枢神经系统具有保护作用。更多还原

【Abstract】 AIM: To explore the molecular mechanism of brain tissue injury induced by endotoxin. METHODS: Rats were divided into LPS, LPS+DEX, LPS-PDS and control group, respectively. NOS activity, NO content and TLR4 mRNA expression were assayed 4 h after intravenous injection of LPS. RESULTS: NOS activity, NO content and TLR4 mRNA expression in LPS + DEX and LPS + PDS groups were obviously lower than those in LPS group. CONCLUSION: PDS may provide protective effects on the central nervous system by down - regulating TLR4 expression, reducing NOS activity and NO content in the brain tissue.更多还原