【摘要】 目的:制备宫颈癌抗独特型单克隆抗体并鉴定其抗原模拟特性。方法和结果:以识别小鼠和人宫颈癌相关抗原分子共同表位的单克隆抗体AU14-1(AB1)为免疫原在含免疫反应剂的无血清培养液中致敏小鼠脾细胞, 并将其与SP2/0融合,经筛选和克隆化,建成一株能分泌抗独特型单克隆抗体(AB2)的杂交瘤细胞系。AB2的抗原模拟特性经ELISA、结合和竞争抑制试验以及免疫组化染色,表明为AB2Β具有宫颈癌细胞膜表面抗原的“内影像”。结论:获得一株具有宫颈癌抗原内影像的抗独特型单克隆抗体。更多还原

【Abstract】 AIM: To produce monoclonal anti-ioliotypic antibody against uterine cervical cancer and identify its anti-genie mimicry properties. METHODS and RESULTS: Mouse splenocytes were immunized in serum - free medium containing immunoactive reagents by using monoclonal antibody AU14-1 (Ab1) recognizing common epitopes shared with both mouse and human uterine cervical cancer-associated antigen molecule as immunogen. The in vitro immunized lymphocytes were fused with murine myeloma SP2/0. Hybridoma cell line secreting monoclonal anti-idiotypic antibody (Ab2) was established by screening and cloning. The identification of antigenic mimicry properties of Ab2 with ELBA, combinding and competing inhibition assays, and immunohistochemistry staining showed that this monoclonal anti-idiotypic antibody was Ab2β, which carried an internal image of cervical cancer cell membrane surface antigen. CONCLUSION: A monoclonal anti - idiotypic antibody with an "internal image" of cervical cancer antigen was obtained.更多还原