【摘要】 目的:构建pEgr-IL18*B7．1双基因表达质粒,探讨不同剂量电离辐射诱导下的表达特性,研究基 因放射治疗对恶性黑色素瘤的抑瘤作用。方法:利用基因重组技术构建包含小鼠IL-18和B7．1的双基因表达质粒 pEgr-IL18-B7．1,分别利用ELISA和流式细胞仪检测不同剂量电离辐射诱导下IL-18和B7-1在体外的表达特性, 以移植肿瘤的生长为判断指标,探讨基因放射治疗对恶性黑色素瘤的抑瘤作用。结果:体外实验证实,双基因表达 质粒pEgr-IL18-B7．1在不同剂量X射线诱导下可有效表达IL-18和B7-1,与放疗联合应用可有效抑制肿瘤生 长。结论:说明多基因联合辐射治疗是有效的抗肿瘤措施之一。更多还原

【Abstract】 AIM: Plasmid Egr-IL18-B7.1 was constructed to explore its expression characteristics induced by different doses of radiation and its suppressive effect on melanoma under radiotherapy.METHODS: The plasmid containing both IL- 18 and B7.1 genes downstream of Egr - 1 was constructed using gene recombination technique. The in vitro expression of IL - 18 and B7.1 induced by ionizing radiation was measured with ELISA and flow cytometry, respectively. The effect of gene radiotherapy on malignant melanoma was assayed by observing the growth rate of B16 cells implanted into C57BL/6J mice. RESULTS: Effective expression of IL-18 and B7.1 by plasmid Egr - IL18 - B7.1 treated with different doses of X - irradiation were observed and in vivo experiments showed significant inhibition of tumor growth after combined gene - radiotherapy. CONCLUSION: Data presented in this paper implicate that gene radiotherapy with plasmid containing double genes might be one of the effective anticancer therapeutic measures.更多还原