【摘要】 目的探讨CpG寡核苷酸(CpGODN)对B淋巴细胞的靶向性及免疫增强效应。方法制备CD40配体(CD40L)-多聚左旋赖氨酸(PLL)-CpGODN交联复合物。复合物与脐血单个核细胞(MNCs)共培养,流式细胞仪检测MNCs对羧基荧光素FAM标记CpGODN的摄取率、淋巴细胞亚群的表达;MTT法测MNCs增殖情况;ELISA法测培养上清IgG水平。结果与CpGODN相比,MNCs对CD40L-PLL-CpGODN交联复合物摄取率更高(>98%),达摄取峰值时间更早,细胞内荧光强度维持更稳定;MNCs与之共培养后CD19+、CD22+、CD20+细胞表达明显升高,MTT法测MNCs增殖情况、ELISA法测培养上清IgG水平均明显升高。结论CD40L-PLL载体系统能将CpGODN靶向作用于B细胞,增强其免疫效应。更多还原

【Abstract】 AIM: To explore the enhancement of CpG oligodeoxynucleotide (CpG ODN) delivery targeting to B lymphocytes and its immunologic efficacy. METHODS: CD40 ligand-PLL-CpG ODN conjugated complex was prepared. Mononuclear cells (MNCs) from umbilical cord blood were cultured with CD40 ligand-PLL-CpG ODN conjugated complex. Uptake rates of FAM labeled CD40 ligand-PLL-CpG ODN complex and subgroups of lymphocytes were analyzed by cytometry. Proliferation of MNCs as detected by MTT assay, and IgG levels of culture supernatants were measured by ELISA. RESULTS: Compared with CpG ODN group, uptake rates of CD40 ligand-PLL-CpG ODN by MNCs were higher (>98%), the peak level of uptake occurred earlier, intracellular fluorescence intensity maintained much more stable. Expressions of CD19+, CD22+, CD20+ increased significantly. A_~570 values of MNCs proliferation and IgG levels in supernatant were all higher. CONCLUSION: CD40 ligand-PLL carrier system may delivery CpG ODN targeting to B lymphocytes, enhancing its immunological efficiency.更多还原