【摘要】 猿猴空泡病毒40(Simian vacuolating virus 40,SV40) 属于乳多空病毒科,是一种DNA肿瘤病毒。亚洲猿类特别是恒河猴是SV40的天然宿主。感染SV40病毒可导致猴体急性病变或呈长期带毒状态,此外能诱使幼鼠产生肿瘤,并能使多种培养细胞发生转化。本研究初步建立了SV40 病毒在Vero细胞中的增殖培养方法,并且初步建立了β丙内脂灭活病毒的方法和纯化工艺。使用SV40病毒灭活疫苗对Balb/c小鼠进行了免疫,结果表明该疫苗具有较好的免疫原性。随后对SV40 病毒DNA在免疫小鼠的重要脏器中的整合情况进行了调查,结果表明SV40病毒DNA未在小鼠重要脏器中整合。本研究为SV40病毒灭活疫苗的研制和进一步开展猴体抗SV40 感染实验奠定了良好的基础。更多还原

【Abstract】 Simian vacuolating virus 40(SV40)belongs to polyoma virus and is a DNA tumor virus.The natural hosts for SV40 are species of Asian macaque monkeys, especially the rhesus (Maccaca mulatta). SV40 can cause widespread infections leading to acutely pathological changes and long-term virus schleping in healthy monkeys. SV40 was shown to be tumorigenic in rodents and was able to transform many types of cells in culture. This work represented the first description of inactivated SV40 by beta-propiolactone and evaluated its immunogenic properties in mice. This work was a primary attempt to tackle SV40 infection and spread has made it ready to further investigate the immunological characteristics of the inactivated virus in monkeys and provided a foundation for vaccine development against SV40.更多还原