【摘要】 目的:为使间充质干细胞(MSCs)被绿色荧光蛋白(GFP)稳定标记,探讨通过逆转录病毒载体介导的GFP基因高效转染MSCs的方法,以便为研究在体干细胞分化奠定基础。方法:全骨髓法分离培养纯化大鼠MSCs,利用携带GFP基因的复制缺陷性逆转录病毒(R t-GFP)转染MSCs,荧光显微镜观察GFP阳性的MSCs。结果:R t-GFP转染后,80%~90%MSCs可激发出明亮的绿色荧光,且荧光不随培养时间延长和传代次数增加而衰减。结论:R t-GFP可以使MSCs获得长效稳定标记。更多还原

【Abstract】 Objective To obtain stable labelling of mesenchymal stem cells(MSCs) mediated by recombinant replication-deficient retroviral vector expressing green fluorescent protein(Rt-GFP) and provide a basis for in vivo differentiation study of MSCs in future.Methods Rat MSCs were cultured and purified with whole bone marrow method.The purified MSCs were infected with Rt-GFP.The GFP positive MSCs were observed with fluorescent microscopy.Results After Rt-GFP infection,80%~90% MSCs were labelled by GFP.The fluorescent intensity of labelled MSCs was not changeable with the prolonged culture time and the increase of passage.Conclusion MSCs may be stably labelled by Rt-GFP.更多还原