【摘要】 利用AFLP技术对甜瓜属人工异源四倍体(Cucum is×hytivusChen and K irkbride,2n=38)不同自交世代及二倍体亲本DNA进行分析,结果表明多倍体基因组在形成早期发生了广泛的变化,变化位点所占比例高达22.2%。基因组序列变化始于F1代,最高变化率出现在S2代。基因组变化主要表现为部分亲本片段丢失、新片段产生以及后代对亲本某些位点的跳跃式继承。还观察到部分双亲差异性条带呈现出偏父性遗传的特点。更多还原

【Abstract】 AFLP analysis was carried out in amplification of the DNAs from different generations of the synthetic allotetraploid（Cucumis×hytivus,Chen and Kirkbride,2n=38）,the interspecific F₁,and its diploid parents.The results indicated that extensive genomic changes occurred in the synthetic allotetraploid,and the variation rate was as high as 22.2%.We found the genomic changes started in the F₁ and the highest variation rate observed in S₂.Most genomic changes involved the loss of parental fragments,formation of novel fragments,with some fragments disappearing in one generation and reappearing in others.In addition,it was observed that some parent-specific bands were inherited preferentially from the male parent.更多还原