【摘要】 从土壤中分离到对黄瓜枯萎病病原菌有抑制作用的枯草芽孢杆菌(Bacillus subtilis),发酵产物经DEAE-纤维素、Sephadex G-100柱层析,分离到15 kD抑菌蛋白L37,SDS-PAGE显示纯度达到电泳纯,N-末端氨基酸序列同源性检测结果提示可能是一种新型的抑菌蛋白。蛋白质抑菌结果表明,对多种病原真菌具有抑制效果,且对蛋白酶、温度和大部分有机物不敏感,是稳定的抑菌蛋白。对病原菌菌丝抑制机理研究表明,抑菌蛋白具有严重扭曲、断裂、阻止生长等抗生效果。应用发酵蛋白产物进行两年大田防效试验表明,对黄瓜枯萎病防效显著,最高达到90%,具有显著提高产量的作用。更多还原

【Abstract】 Bacillus subtilis inhibiting cucumber wither pathogen was screened from soil.After fermenting,15 kD antagonistic protein named L37 was got through DEAE-fibrin,Sephadex G-100 chomatography,which proved purified completely through SDS-PAGE.It was probably a new type of antagonistic protein against pathogen by the sequence on the amino N-terminal testing,which could make the normal hypha of pathogen distort,split and inhibit.The control effect could reach 90% against cucumber wither pathogen and improved the yield in field controlling test by fermenting products.更多还原