【摘要】 利用常规石蜡切片技术对黄花菜受精过程进行了详细的研究,获得如下结果:1.授粉后1~2 h花粉在柱头上萌发长出花粉管,只有生殖细胞进入花粉管内。2.花粉管在花柱道内生长历经20~22 h,生殖细胞分裂形成2个精子。3.花粉管在子房室中轴胎座表面生长经历2~4 h。4.授粉后26~28 h花粉管进入1个助细胞,释放2个精子。5.精卵融合经历4~6 h,形成合子。6.精核与次生核融合经历2~4 h,形成初生胚乳核。7.自花粉在柱头萌发至合子形成,受精过程持续总时间为29~36 h。8.合子分裂发生在授粉后39~48 h。9.讨论了研究结果对黄花菜遗传育种与分子育种的意义。更多还原

【Abstract】 Observed the process of fertilization in Hemerocallis citrina Baroni in detail by means of paraffin wax.The main results are as follows:1.After artificial pollination 1-2 hours,pollens germinated at the top of stigma,growing out pollen tube,where only generative cell can get into.2.Pollen tube prolongs in stylar canal for 20-22 hours,in concomitant with generative cell dividing into two sperms.3.Pollen tube prolongs on the surface of axile placenta in locule for 2-4 hours.4.After artificial pollination 26-28 hours,pollen tube get into one of synergids,and releases two sperms.5.It takes 4-6 hours to eggsperm fusion,and then bring out zygocyte.6.The formation of primary endosperm nucleus results from 2-4 hours’ egg-secondary nucleus fusion.7.The duration of the whole fertilization is 29-36 hours from pollen germination to the formation of zygocyte.8.After artificial pollination 39-48 hours,zygocyte begins to divide.9.Discussed the significance of the result of study to genetic breeding and molecular breeding.更多还原