节点文献
桂花离体培养与快速繁殖技术的初步研究
Primary Study on in Vitro Culture and Micropropagation of Sweet Osmanthus
【摘要】 以桂花(O smanthus fragransLour.)胚和新梢茎段为外植体进行了离体培养与快速繁殖研究,筛选出最适培养基———(1)胚萌发:MS+BA 1.00 mg.L-1+蔗糖3%;(2)新梢茎段启动培养:LM c+KT 8.00 mg.L-1+NAA 0.10 mg.L-1+蔗糖3%;(3)继代增殖培养:LM c+TDZ 0.50 mg.L-1+NAA0.10 mg.L-1+蔗糖3%;(4)生根培养:1/2 MS+NAA 2.00 mg.L-1+蔗糖3%。采用腐殖质土为栽培基质,移栽成活率可达80%以上。
【Abstract】 Using the embryo,stem-segment of new shoots as the explants, in vitro culture and high frequency propagation of sweet osmanthus were studied.The results showed that the optimizing media for various stages were as follows:(1)Initial medium for embryo: MS+BA 1.00 mg·L-1+3% sucrose;(2)Initial medium of stem-segment for new shoots: LMc+KT 8.00 mg·L-1+NAA 0.10 mg·L-1+3% sucrose;(3)Clump shoot regeneration medium: LMc+TDZ 0.50 mg·L-1+NAA 0.10 mg·L-1+ 3% sucrose;(4)Rooting medium: 1/2 MS+NAA 2.00 mg·L-1+3% sucrose.Using humus soil as culture substrate,the survival percentage of plantlets could reach 80%.
- 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2005年04期
- 【分类号】S685.13
- 【被引频次】42
- 【下载频次】520