【摘要】 对河北张家口感病马铃薯的一个马铃薯Y病毒分离物(PXYHBEI)的外壳蛋白基因进行了克隆和序列分析。以提取的RNA为模板,应用RTPCR扩增目的基因,扩增产物克隆到质粒pGEMT,上并序列分析。结果表明,克隆cDNA片段由801个核苷酸组成,编码267个氨基酸。与基因库中PVY代表株系相比,它们的核苷酸和氨基酸序列同源性分别为89.6%～98.8%和93.3%～98.5%,与PVYN和PVYO株系的氨基酸序列同源性分别为93.6%和97.8%,确定PVYHBEI归属于普通株系(PVYO株系),同时建立了快速、灵敏、简便的PVYRTPCR检测方法。更多还原

【Abstract】 A potato virus Y isolate(PVY-HBEI)was obtained from infected potato plants cultivated in Zhangjiakou City of Hebei Province.The coat protein gene (CP) was amplified from the extracted plant total RNA by using RT-PCR, and cloned into the pGEM-T vector and sequenced. The cloned segment was 801 bp and encodes 267 amino acid residues.It was cpmpared with other PVY isolates reported in GenBank.It shares 89.6%-98.8% and 93.3%-98.5% homology in nucleotide and the putative amino acid sequence,respectively,which suggested that the CP gene of PVY isolates is a relatively conserved sequence within the PVY genome,the homology between PVY-HBEI and PVY N and PVY O were 93.6% and 97.8%,resepectivly,thus it was identified as an isolate of PVY O ,the experiment also provided a rapid,sensitive and relatively inexpensive methods of PVY RT-PCR detection.更多还原