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茄子抗青枯病基因的RAPD标记研究
Studies on RAPD Marker of Bacterial Wilt Resistance Gene in Eggplant(Solanum melongena)
【摘要】 为探明茄子抗青枯病的遗传机制,以高感青枯病品种北京六叶茄064,高抗青枯病半栽培种马来西亚S3及其F2代为材料,用RAPD技术对供试材料的抗青枯病基因进行了研究。结果表明:通过300个随机引物的PCR扩增分析,发现15.6%的引物在双亲间表现出多态性,找到了一个与茄子抗青枯病亲本S3中的抗病基因紧密连锁的分子标记S264780(该引物的碱基序列为CAGAGCGGA),该标记与S3的抗病基因的交换值为4.32%,遗传距离为4.33cM。
【Abstract】 To probe into the genetic resistant law to bacterial wilt(BW)in eggplant,the genetic studies to BW of 064(Beijing six-leaf eggplant),S3( highly resistant half-cultivated species of Malasia) and 064×S3 F2 populations is carried out on RAPD marker.300 decamer primers with arbitrary sequences are chosen for polymerase chain reaction amplication.It is found that the polymorphism between the parents could be detected by 15.6% of the primer; one RAPD marker S264 780 (the sequence of the primer:CAGAAGCGGA) tightly links to the resistance gene of S3 with the overcross value of 4.32% and genetic distance of 4.33 cM.
- 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2005年02期
- 【分类号】S641.1
- 【被引频次】39
- 【下载频次】334