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苦瓜中降糖物质的提取研究
Study on the extraction of lowering blood sugar substance from momordica charantia L
【摘要】 采用有机溶剂浸提法从苦瓜中提取了降糖物质。通过正交实验得最佳反应条件为:浸提剂乙醇浓度75%,温度0.5℃,pH值3.0,时间24h。该条件下收率达新鲜苦瓜质量的0.87%。红外光谱定性分析验证了产品为蛋白类物质;HPLC定性分析表明产品中含有三至四种蛋白类物质。
【Abstract】 The extraction conditions of lowering blood sugar substance from mormordica charantia L were optimized by orthogonal design.The result showed the optimized conditions were as follows: extraction pH 3.0,extraction temperature 0.5 ℃,extraction time 24h,extraction solvent 75%.The recovery is 0.87% under the optimized condition.IR qualitative analysis well tested and verified the output was plant insulin.The HPLC qualitative analysis indicated that there were three or four proteins in the output.
【关键词】 苦瓜;
降糖物质;
提取;
【Key words】 mormordica charantia L; lowering blood sugar substance; abstraction;
【Key words】 mormordica charantia L; lowering blood sugar substance; abstraction;
- 【文献出处】 湖南理工学院学报(自然科学版) , 编辑部邮箱 ,2005年04期
- 【分类号】TQ464
- 【被引频次】3
- 【下载频次】209