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苦瓜中降糖物质的提取研究

Study on the extraction of lowering blood sugar substance from momordica charantia L

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【作者】 陈国斌唐课文曹金艳

【Author】 CHEN Guo-bin,TANG Ke-wen,CAO Jin-yan(Department of Chemistry and Chemical Engineering,Hunan Institute of Science and Technology,Yueyang 414000,China)

【机构】 湖南理工学院化学化工系湖南理工学院化学化工系 湖南岳阳414000湖南岳阳414000

【摘要】 采用有机溶剂浸提法从苦瓜中提取了降糖物质。通过正交实验得最佳反应条件为:浸提剂乙醇浓度75%,温度0.5℃,pH值3.0,时间24h。该条件下收率达新鲜苦瓜质量的0.87%。红外光谱定性分析验证了产品为蛋白类物质;HPLC定性分析表明产品中含有三至四种蛋白类物质。

【Abstract】 The extraction conditions of lowering blood sugar substance from mormordica charantia L were optimized by orthogonal design.The result showed the optimized conditions were as follows: extraction pH 3.0,extraction temperature 0.5 ℃,extraction time 24h,extraction solvent 75%.The recovery is 0.87% under the optimized condition.IR qualitative analysis well tested and verified the output was plant insulin.The HPLC qualitative analysis indicated that there were three or four proteins in the output.

  • 【文献出处】 湖南理工学院学报(自然科学版) , 编辑部邮箱 ,2005年04期
  • 【分类号】TQ464
  • 【被引频次】3
  • 【下载频次】209
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