【摘要】 采用悉生培养微缩体系,探讨了不同食细菌线虫取食密度下线虫(Caenorhabditiselegans)对细菌(Bacillussubtilis)数量和活性及土壤氮素矿化的影响.结果表明,线虫对细菌的取食,促进了细菌的增殖,并在不同线虫取食密度下对细菌的增殖促进作用总体表现为:接种20条·g-1>10条·g-1>40条线虫·g-1处理.线虫在促进细菌增殖的同时,明显提高了土壤呼吸强度和土壤蔗糖酶、脲酶和磷酸酶的活性,但不同取食密度处理间差异不明显.线虫与细菌之间的相互作用显著提高了土壤铵态氮和矿质态氮含量,促进了土壤氮的矿化.不同取食密度处理间,线虫对土壤氮素矿化的促进作用与对细菌的增殖促进作用趋势一致.更多还原

【Abstract】 A gnotobiotic microcosm experiment was conducted to study the interactions between bacteria-feeding nematode Caenorhabditis elegans and bacterium Bacillus subtilis,and their effects on soil nitrogen mineralization at different Caenorhabditis elegans density.The results showed that the inoculation of the nematode stimulated the growth of the bacterium,and the increment was in order of 20>10>40 nematodes·g^-1 dried soil.The interaction between Caenorhabditis elegans and Bacillus subtilis significantly enhanced soil respiration rate and soil invertase,urease and phosphatase activities,with no significant differences among three test nematode densities.The inoculation of bacterial-feeding nematode markedly increased soil NH₄⁺-N and mineral N,suggesting that soil N mineralization was enhanced under the effect of the nematode.The increment of soil nitrogen mineralization at different nematode density was also in the same order mentioned above.更多还原