【摘要】 通过在饮水中加入硝酸镧使小鼠摄入稀土,1个月后采用ICPMS法测试了镧在小鼠肝中的积累,研究了硝酸镧对骨髓细胞微核率的影响,并采用体外试验研究了硝酸镧对小鼠基因组DNA的切割作用,探讨了稀土元素镧的遗传毒理.结果表明,1000、500、300和50μg·ml-1处理组小鼠肝中镧含量分别达146、0558、0529和0083μg·g-1,与对照组0028μg·g-1的含量相比,各处理组小鼠肝中镧的含量皆有升高且与喂饮硝酸镧溶液的浓度成正比(r=0980).1000、500和300μg·ml-1处理组微核率与对照组之间的t检验结果表明各组微核率显著上升(P<005),且亦与喂饮硝酸镧溶液的浓度成正比(r=0853).体外试验显示,硝酸镧可切断DNA链,说明稀土元素镧可在生物体内积累,其在细胞内可使DNA断裂而导致遗传物质受损.更多还原

【Abstract】 By using ICP-MS method,this paper determined the accumulation of La in mice liver after the mice being fed with La（NO₃）₃ water solution for thirty days,and through in vitro experiment,studied the effect of La（NO₃）₃ on the micronucleus rate of mice bone marrow cells and the cleavage action of La（NO₃）₃ on genome DNA to investigate the genetic toxicity of La.The results showed that when the treated concentration was 1 000,500,300 and 50 μg·ml^-1 ,the amount of La in mice liver reached 1.46,0.558,0.529 and 0.083 μg·g^-1 ,respectively.Compared with control,the La amount in disposed groups’ mice livers increased with increasing La（NO₃）₃ concentration in water（r=0.980）.T-test results showed that there existed significant differences in 1 000,500 and 300 μg·ml^-1 disposed groups when compared with the control（P<0.05）.The micronucleus rate of mice bone marrow cells increased with increasing La（NO₃）₃ concentration in water（r=0.853）.The in vitro experiments showed that La（NO₃）₃ could make DNA cleaved.It could be concluded that that La might be accumulated in organisms,and could induce the damage of genetic material in cells.更多还原