【摘要】 为了阐明已分离的盐碱适应性相关基因(Liuetal, 2000)的表达特性,以14d叶龄的水稻品种日本晴幼苗为材料,分别经NaHCO3 (60mmol/L,pH8. 50) )处理6h、12h、24h、48h, 及不同pH值(pH4. 50, 6. 50, 8. 50, 10. 50)的NaCl(100mmol/L), NaHCO3 (60mmol/L)和Na2CO3 (30mmol/L)处理24h, 以分析时间效应及浓度与pH值间的联合效应. 结果表明,与对照(H2O, pH6. 50)相比较,NaHCO3 (60mmol/L)处理6h、12h、24h、48h后,mitochondrialAT Pase6×103的转录本分别提高2. 73、0. 82、0. 01和1. 79倍;cAPX转录本在24h和48h较对照提高1. 17和1. 49倍;处理6h后CA和HSP90基因表达受显著抑制;UDPG-GT增强表达的滞后期为6h;thioredoxin受NaHCO3轻微抑制.不同pH值和24h处理条件下,NaHCO3和Na2CO3显著促进mitochondrialATPase6×103基因表达.CA基因表达受Na+、HCO-3 和pH的共同影响;而thioredoxin主要受Na+和pH的双重影响; cAPX也表现出类似趋势.UDPG-GT在Na2CO3处理中表达量最高,HSP90的表达随pH的升高而降低. 图6表2参13更多还原

【Abstract】 To characterize both individual and combinational effects of salt, alkaline concentration and different pH values on six salt and alkaline stress-related gene s isolated via DDRT-PCR from rice by Liu et al (2000), fourteen-day-old seedlings of Nipponbare (Oryza sativa L. subsp. japonica ) were exposed to NaHCO 3 (60 mmol/L, pH 8.50) in 6 h, 12 h, 24 h, 48 h, as well as to NaCl (100 mmol/L), NaHCO 3 (60 mmol/L) and Na 2 CO 3 (30 mmol/L) in 24 h with pH values of 4.50, 6.50, 8.50 and 10.50, followed by Northern blot analysis in the shoot. The results showed that, compared with that treated by H 2 O (pH 6.50), mitochondrial ATPase 6×10 3 subunit was 2.73, 0.82, 0.01, 1.79 fold higher in 6 h, 12 h, 24 h, 48 h by NaHCO 3 (60 mmol/L, pH 8.50). The transcription of HSP90 and CA were significantly repressed in 6 h later, transcript of UDPG-GT was enhanced in 6 h, but expression of thoredoxin was slightly repressed under stress treatment condition. Under 24 h and different pH value treatments, NaCl (60 mmol/L, pH 8.50), NaHCO 3 and Na 2 CO 3 treatments between acidic (pH 4.50 ) and alkaline (pH 8.50 and 10.50) obviously increased the expression of mitochondrial ATPase 6×10 3 subunit , CA was coordinately influenced by salt, alkaline concentration and pH values, thioredoxin and cAPX were mainly affected by salt and pH, and UDPG-GT transcript reached the peak in Na 2 CO 3 treatment. And low pH value increased HSP90 expression, however high pH could repress its expression.Fig 6, Tab 2, Ref 13更多还原