【摘要】 目的通过体外实验研究雌激素、孕激素和肝素结合表皮生长因子(HB-EGF)对子宫内膜癌细胞中子宫内膜容受性的标志整合素β3(integrinβ3)表达的调节,探讨整合素β3表达的意义及子宫内膜容受性的调节因素。方法体外培养高分化子宫内膜癌细胞Ishikawa,按实验目的分别加入雌激素、孕激素、雌孕激素联合、雌孕激素及孕酮抑制药(RU486)、HB-EGF刺激48h后,采用逆转录-聚合酶链反应(RT-PCR)、Westernblot、原位杂交、免疫细胞化学方法测定各个条件下内膜癌细胞中整合素β3mRNA及蛋白表达。结果雌激素单独以及雌激素、孕激素同时作用均使Ishikawa细胞中整合素β3mRNA及蛋白的表达显著下降;RU486可减弱雌激素、孕激素对integrinβ3的下调作用;而孕激素单独应用对整合素β3表达无明显影响;HB-EGF可显著提高整合素β3的表达。结论整合素β3mRNA及蛋白的表达受甾体激素和生长因子两方面的调节。更多还原

【Abstract】 Objective To study the regulation of the integrin β3 expression in a well differentiated endometrial adenocarcinoma cell line （Ishikawa） by estrogen （E₂）, progestin （P 4 ） and heparin-binding epidermal growth factor （HB-EGF）. Methods Ishikawa cells were treated with nothing or with 17β-E₂ （10 -8 mol·L -1 ）, P 4 （10 -6 mol·L -1 ）, HB-EGF （10 ng·mL -1 ）, 17β-E₂ plus P 4 , 17β-E₂ plus P 4 and antiprogestin （RU 486 10 -5 mol·L -1 ） for 48 hours. The expression of the integrinβ3 was determined with reverse transcription-polymerase chain reaction （ RT-PCR）, western bolt analysis, in situ hybridization, and immunocytochemistry. Results The expression of integrin β3 was significantly reduced by 17β-E₂ treatment or by 17β-E₂ plus P 4 treatment. The addition of mifepristone RU486 reversed the suppression of integrin β3. P 4 had no significant effect on integrin β3 expression. HB-EGF significantly increased the integrin β3 expression. Conclusion The expression of the imtegrin β3 is regulated by both steriod hormones and growth factors.更多还原