【摘要】 目的　研究大黄素对去卵巢大鼠和经 β淀粉样蛋白 (βamyloidprotein ,β AP)作用的培养神经元的保护作用。方法　①将 2 9只Wistar大鼠随机分为 3组 :模型组 10只 ,手术切除双侧卵巢后饲喂普通食物 ;给药组 10只 ,手术切除双侧卵巢后饲喂含大黄素的食物 ,大鼠摄入大黄素按 80 μg·kg 1 ·d 1 计 ;对照组 9只 ,给予假手术后饲喂普通食物。70d后通过水迷宫实验测定 3组大鼠行为改变情况。②取经过上述实验的 3组大鼠的脑额叶皮质细胞 ,以流式细胞仪检测早期凋亡细胞百分数。③取乳鼠大脑皮质细胞 ,培养 12d后分为 4组 :实验组 1加入 β AP1 4 0 ;实验组 2加入 β AP1 4 0 和 17 β雌激素 ,实验组 3加入β AP1 4 0 和大黄素 ;正常组不加入任何药物。观察 4组培养细胞的早期和晚期存活数 ,测定标记Annexin V FITC的细胞的荧光强度。结果　①给药组和对照组大鼠比模型组大鼠更容易找到实验平台的位置。给药组和对照组到达平台的平均时间分别为 (15 19± 2 2 4)、(14 48± 1 99)s ,均比模型组大鼠到达平台所用的平均时间 [(2 2 45± 2 42 )s]短 (P <0 .0 1) ;给药组和对照组到达平台的游泳距离分别为 (4 3 2 91± 2 99)、(4 2 6 5 8±2 66)cm ,均比模型组到达平台的游泳距离 [(766 66± 3 5 7)c更多还原

【Abstract】 Objective To study the influence of emodin on the behavior of ovariectomized rats and its protective effect on cultured neurons of these rats and those murine neurons treated with β-amyloid protein(β-AP). Methods ① Twenty-nine adult female Wistar rats were randomly divided into 3 groups: 10 animals of the model group were submitted to bilateral ovariectomy and fed on conventional chow, 10 rats of the test group were ovariectomized and fed on chow containing (80 μg·kg -1) of emodin·d -1. 9 rats of the control group underwent a sham operation without removing the ovaries and were fed on conventional chow. Succeeding experiments began after rats of all 3 groups had been bred and treated for 70 days. The behavior of the rats of all 3 groups was assessed with the Morris water maze (MWM) test. ② Early apoptosis of cerebral cortical cells from the frontal lobes of all of the rats that had been subjected to experiment ① was assayed with flow-cytometry. ③ Cerebral cortical cells of suckling rats were incubated for 12 d and then divided into 4 groups. Cells of test group 1 were treated with β-AP 1-40 ;cells of test group 2 were treated with β-AP 1-40+17-β-estradiol ;cells of test group 3 were treated with β-AP 1-40+emodin ;Cells of group 4 served as normal controls. Percentages of the early and late stage viable cells of all of the 4 groups were calculated and the fluorescence intensity of the cells labelled with Annexin V-FITC was determined. Results ① Rats of the test group and control group were shown to find the place of the escape platform more readily than those of the model group .The swimming times for the rats of the test group and control group to reach the escape platform were(15.19±2.24)s and (14.48±1.99)s,respectively, which were significantly shorter than the time for rats of the model group to reach the platform-(22.45±2.42)s, P<0.01. The swimming distances for rats of the test group and control group to reach the escape platform were (432.91±2.99)cm and (426.58±2.66)cm, respectively, which were significantly shorter than the distance for the rats of the model group to reach the platform –(766.66±3.57)cm, P<0.01. ②The percentages of apoptotic cerebral cortical cells from rats of the test group,control group and model group were (5.4±0.7)%,(5.3±0.7)% and (12.8±2.5)%,respectively.The differences between the former 2 values and the latter were both significant(P<0.01).③The growth of neurons and their neurites of the test group 1 was inhibited and the percentages of early apoptosis of cells in this group was higher than those percentages of the test group 2, test group 3 and test group 4(control).The fluorescence intensity of Annexin-V-FITC labelled cells of the test group 1(6.19±0.27) was significantly stronger than those of test group 2(4.3±0.29),test group 3(4.8±0.9) and test group 4 (4.1±0.11), respectively. Conclusion Emodin was shown to exert beneficial influence on ovariectomized rats and cultured rat neurons treated with β-AP 1-40.更多还原