【摘要】 目的应用我国自行研制的无标记光学蛋白质芯片检测乳腺癌组织中的胸苷磷酸化酶(Thymidine phosphorylase,TP),为建立一种快速、简便的临床检测方法进行初步探索。方法将 TP 的多克隆抗体固定于已经过表面改性的固相光学抛光硅片表面上制备无标记光学蛋白质芯片,通过椭偏光学显微成像技术检测20例经病理证实的乳腺浸润性导管癌组织标本和正常组织标本中 TP 水平,以芯片灰度值表示组织中 TP 浓度高低。同时应用 ELISA 方法测定,并比较两种方法检测结果的一致性。结果 20例癌组织的灰度值35.40±11.55,20例正常组织的灰度值为26.80±8.78,两组间差异具显著性意义(P<0.05)。以44.36(正常组织平均值+2倍标准差)为阈值,乳腺癌检测的灵敏度为20.00%,特异度为100.00%。同 ELISA 检测结果相比,在20例乳腺癌组织标本检测中,两种方法的一致性具极显著意义(P<0.01)。结论我国首创的椭偏光学显微成像技术简单、直观,结合光学蛋白质芯片可用于胸苷磷酸化酶的临床检测。更多还原

【Abstract】 Objective Unlabeled Optical Protein Chip(OPC)created in CAS(Chinese Academy of Science)was initially used to measure the level of thymidine phosphorylase(TP)in invasive ductal breast cancer in order to set up a fast and simple clinical metliod.Methods The level of TP in 20 cases of and 20 cases of healthy tissue verified by histology detection was measured using a kind of OPC on which anti-TP was fabri- cated on the surface of solid optical chip,then the result was showed directly by Imaging Ellipsometry system in gray way and compared with ELISA assay at the same time.Resuhs The gray of TP in breast tissue was 35.40±11.55 and 26.80±8.78 in healthy tissue with a significantd- ifference between them(P<0.05).According to the cutoff gray 44.36(mean+2SD),the sensitivity of TP in breast cancer was 20.00% and speciality was 100.00%.And there is a significant concordance between OPC detection and ELISA assay(P<0.01)among the 20 cases of can- cer tissue.Conclusion The Imaging Ellipsometry method combining OPC is simple,fast and visual that might enable its good clinical feasibility in TP detection.更多还原