【摘要】 目的研究双苯氟嗪对大鼠脑缺血再灌注后E-选择素(E-selectin)、P-选择素(P-selectin)和细胞间黏附分子-1(ICAM-1)表达及中性粒细胞浸润的影响。方法采用Zea-Longa线栓法建立大鼠局灶性脑缺血再灌注模型。采用HE染色、免疫组化、流式细胞术以及生化的方法,观察双苯氟嗪对大鼠缺血再灌注后脑组织形态学、P-选择素、E-选择素和ICAM-1表达以及髓过氧化酶(MPO)活性的影响。结果双苯氟嗪可改善缺血再灌注后脑损伤的形态学表现;降低P-选择素、E-选择素和ICAM-1的表达以及MPO的活性。结论双苯氟嗪减轻缺血再灌注后炎症反应,对缺血再灌注性脑损伤具有保护作用。更多还原

【Abstract】 Aim To evaluate the effects of dipfluzine on the expressions of E-selectin,P-selectin,and ICAM-1 and the infiltration of polymorphonuclear leukocytes in brain ischemia-reperfusion rats.Methods The model of focal cerebral ischemia was established with the Zea-Longa occluding suture.Dipfluzine(0.25,0.5 and 1 mg·kg^-1),flunarizine 0.5 mg·kg^-1 and solvent were injected separately into lingual vein at 30 min after ischemia.The occluding suture was slowly taken away to cause reperfusion at 1 h after ischemia.Rats were decapitated under anesthesia at 24 h after ischemia-reperfusion and brains were immediately removed to do the following procedures.Effects of dipfluzine on morphology of the brain tissue were observed through hematoxylin-eosin（HE） staining.By immunohistochemistry and flow cytometry technique and biochemical method,effects of dipfluzine on P-selectin,E-selectin,ICAM-1 and myeloperoxidase（MPO） were observed.Results Dipfluzine could relieve pathological damages in the brain tissue after ischemia-reperfusion,and reduce the expressions of E-selectin,P-selectin and ICAM-1 and activities of MPO in dose-dependent manner.Conclusion Dipfluzine depresses the expressions of（P-selectin）,E-selectin,and ICAM-1,which are correlated with their effects on the activities of MPO,suggesting that dipfluzine has anti-inflammation effect in certain extent and could protect brain tissue from ischemia-reperfusion injury.更多还原