【摘要】 目的建立简易的采用rDNAITS区作为分子标记对中药石斛的基源植物进行分子鉴定的技术。方法用聚合酶链反应—限制性片段长度多态性(PCRRFLP)方法获得ITS片段的限制性图谱。束花石斛及其形态相似种的PCR扩增产物用ClaI和ApaLI酶切,流苏石斛及其形态相似种的PCR扩增产物用SphI酶切。结果根据预测的PCRRFLP图谱,可以鉴别药用植物束花石斛、流苏石斛及它们的形态相似种。用这种方法鉴定了市场上收集的25件商品束花石斛、流苏石斛新鲜药材的原植物。结论rDNAITS的PCRRFLP可用于鉴定石斛药材的原植物,具有简便、费用低的优点。更多还原

【Abstract】 Aim To establish a simple method for molecular identification of original plants of D.chrysanthum and D.fimbriatum using molecular marker rDNA ITS region. Methods Restriction patterns of ITS fragments were obtained using PCR-RFLP method. The PCR products of D.chrysanthum and its morphologically allied species were digested at 37 ℃ by Cla I and Apa LI, those of D.fimbriatum and its morphologically allied species were digested by Sph I. Results D.chrysanthum, D.fimbriatum and their morphologically allied species could be identified by predicted restriction profiles of PCR-RFLP. The botanical origin of twenty-five fresh samples of “Shihu” collected in markets was identified by this method. Conclusion The results showed that PCR-RFLP analysis of the rDNA ITS region is a feasible, simple and inexpensive method for determining the botanical origin of the traditional Chinese medicine “Shihu”.更多还原