【摘要】 目的:建立知母药材中知母皂苷BⅡ的 TLC 鉴别及 HPLC 含量测定方法。方法:TLC 法,展开剂:氯仿-甲醇-水（65:35:10）10℃以下放置的下层溶液为展开剂,显色剂:10%硫酸乙醇溶液,105℃加热至斑点显色清晰。HPLC 法,色谱柱:HiQ-sil C₁₈（5μm,4.6mm×250mm）,流动相:甲醇-水（53:47）,流速:0.8 mL·min^-1,检测:蒸发光散射检测器（ELSD）,漂移管温度:100℃,气体流速:2.6L·min^-1。结果:3批药材中知母皂苷 BⅡ成分的 TLC 分离效果均较好,HPLC 法测得知母皂苷 BⅡ的平均回收率为97.7%,RSD 为1.8%。结论:该方法准确可靠,可用于知母药材质量控制。更多还原

【Abstract】 Objective:To establish methods for identification and determination of Anemarrhenasaponin B Ⅱ in Rhizoma Anemarrhenae by TLC and HPLC.Method:The experiment conditions for:Anemarrhenasaponin B Ⅱ in Rhizoma Anemarrhenae was provided by TLC,the mobile phase was chloroform-methanol-water（65:35:10,low- erlayer）.Separation were performed on a HiQ-sil C₁₈ （5 μm,4.6 mm×250 mm） colum of HPLC.The mobile phase consisted of methanol-water（53:47）,the flow rate was 0.8 mL·min^-1.The detector was ELSD.Results: Anemarrhenasaponin B Ⅱ was well separated by TLC and the average recovery of HPLC was 97.7%,RSD was 1.8%.Conclusion:The methods are accurate and can be use for the quality of Rhizoma Anemarrhenae.更多还原