【摘要】 目的:建立用离子抑制-反相高效液相色谱快速测定池杉、落羽杉根系中 L-苹果酸、莽草酸含量。方法:采用 Agilent1100 DAD 检测器,Hypersll ODS2 5 μm（4.6mm×150mm）柱,HClO₄（pH=2.5）溶液为流动相,柱温30℃时于214 nm 波长处以外标法测定。结果:L-苹果酸、莽草酸分别在7.16×10^-6mol·L^-12.24×10^-2mol·L^-1和1.27×10^-7mol·L^-1～2.06×10^-4mol·L^-1范围内,浓度与峰面积有良好的线性关系（r=1.0000和r=0.9999）。检测限分别为2.42×10^-8mol·L^-1和1.16×10^-9mol·L^-1（S/N=3）。结论:该法简便、快速、有效、重现性好,首次用于实际池杉、落羽杉根系中 L-苹果酸、莽草酸的测定,取得了很好的结果。更多还原

【Abstract】 Objective:A ion-suppression reversed-phase HPLC method was established for the determination of rudimental L-malic acid and Shikimic acid in Taxodium aseendems and T axodium distiehum.Methods:They can be separated on Agilent 1100 DAD and Hypersil ODS2 5μm,（4.6 mm×150 mm）column by using perehloric acid （pH=2.25）as mobile phase with UV detector at a wavelength of 214 nm.Results:The standard curve of L-malic acid and shikimic acid was linear in the concentration range 7.16×10^-6mol·L^-1-2.24×10^-2mol·L^-1 and 1.27×10^-7 mol·L^-1-2.06×10^-4mol·L^-1and the correlation coefficient was 1.0000 and 0.9999.The detec- tion limit was 2.42×10^-8 mol·L^-1 and 1.16×10^-9 mol·L^-1（S/N=3）.Conclusions:The method has been suc- cessfully applied to the determination of trace rudimental L-malic acid and Shikimic acid in the root of Taxodium ascendens and Taxodium distiehum,which was proved to be accurate,simple effective and feasible.更多还原