【摘要】 目的:采用LC/MS2方法鉴定出中药红毛五加中绿原酸成分,并用高效液相色谱法测定其含量。方法:采用Zorbax SB-C18色谱柱(150 mm×4.6mm,5μm);流动相为乙腈和水(含0.16%甲酸)二元梯度洗脱系统;流速为1.0mL·min-1,检测波长327 nm。质谱条件:Agilent多级离子阱质谱仪:电喷雾化学电离源(ESI);负离子检出模式。结果:绿原酸的线性范围为 10-100 mg·L-1(r=0.9997),加样回收率为99.2%-101.8%;红毛五加中绿原酸的含量为0.58-5.45mg.g-1。结论:本方法灵敏、快速、重现性好,有助于红毛五加质量控制方法的研究。更多还原

【Abstract】 Objective:Chlorogenic acid in Acanthopanax giraldii Harms was identified by LC/MS . Method:RP -LC method was applied for the determination of chlorogenic acid in Acanthopanax giraldii Harms collected in Sichuan province,China. The separation was performed on a Zorbax SB C18( 150 mm×4.6 mm,5μm) column,and a mobile phase program of gradient elution with acetonitrile and 0.16% aqueous formic acid at a flow rate of 1.0mL·min-1 was employed. The column effluent was monitored at UV 327 nm with diode array detector (DAD). The mass spectrometry analysis was performed on the Agilent 1100 series ion trap mass spectrometer with an electrospray ionization source in the negative ion detection mode. Qualitative identification was carried out by the comparison of the HPLC retention time and the LC/MS2 spectra of chlorogenic acid with corresponding authentic sample. Results:The linear range of chlorogenic acid was 10-100 mg·L-1 (r =0.9997) and the average recovery was 99.2%-101.8% . The content of chlorogenic acid in A. giraldii Harms was 0. 58 -5. 45 mg·g-1. Conclusion:The method is sensitive and rapid, with a good reproducibility. It is useful for the quality control of A. giraldii Harms.更多还原