【摘要】 目的:测定中药三七花中挥发油成分。方法:采用自制的同时蒸馏萃取装置提取(水蒸气蒸馏,乙醚萃取)云南文山三七花的挥发油成分,然后经气相色谱-质谱联用仪测定,用峰面积归一化法定量。分析条件:HP-5石英毛细管柱(30 m×0.25 mm×0.25 μm),程序升温50—250℃,分流进样,分流比50:1,进样温度250℃,MS检测器。结果:挥发油中共分离出91个成分,鉴定了其中53个化合物,主要为萜烯类及其含氧衍生物(71.15%)。含量较高的是匙叶桉油烯醇(12.19%)、α-古芸烯(6.73%)、双环吉马烯(6.57%)、吉马烯-D(5.26%)、双环榄香烯(3.55%)。结论:本方法提取和测定挥发油高效、适用、准确。更多还原

【Abstract】 Objective: To develop the method for the determination of the volatile oil from the flowers of Panax notoginseng(Burk.) F. H. Chen. Method; Gas chromatography-mass spectrum instrument was used to analyze the volatiles, which were extracted by self-made simultaneous distillation and solvent extraction (SDE). Analytic conditions were:capillary column HP-5(30 m×0. 25 mm×0. 25μ0m), temperature program 50-250 °C,split ratio 50: 1,injection temperature 250°C,mass selective detector. Helium was used as carrier gas. Results;It was revealed that 91 peaks have been separated,of which 53 compounds were identified and quantified. The main components are terpenes and their oxo-derivatives(71. 15%). The contents of spathulenol,α—gurjunene, bicyclogermacrene, germacrene-D, bicycloelemene are high. Conclusion; This method is suitable for the extraction and determination of the volatile oils.更多还原