【摘要】 目的:用气相色谱-质谱法对海狗油多不饱和脂肪酸进行成分分析。方法:以精制海狗油为原料,采用毛细管色谱柱进行分离,用归一化法计算含量。色谱条件:HP—INNOWAX毛细管柱（25 m×O.320 mm,0.25μm）,柱温条件:起始温度150℃（1 min）15℃·min^-1→200℃ 2℃·min^-1→250℃（5 min）;分流进样,分流比10:1;进样口温度220℃,MS检测器。结果:海狗油中共鉴定出16个化合物,占海狗油总量的94.19%。主要成分和有效成分为十四碳酸（4.55%）、十六碳酸（8.13%）、十六碳烯酸（17.55%）、油酸（25.75%）、二十碳烯酸（11.19%）、二十碳五烯酸（6.82%）、二十二碳烯酸（2.38%）、二十二碳五烯酸（4.16%）和二十二碳六烯酸（7.94%）。结论:本法可靠,可用于鉴别测定这类产品的真伪。更多还原

【Abstract】 Objective:The polyunsaturated fatty acids in Harp Seal Oil were analyzed by GC-MS.Method:The oil was refined from Harp Seal and analyzed by gas chromatography.The amount of the chemical components was determined by normalization method.Chromatographic conditions were:HP- INNOWAX capillary column（25m×0.320mm,0.25μm）, column temperature:150℃（1min）15℃·min^-1 200 ℃ （0 min）2℃·min^-1 250℃ （5min）,split injection,split ratio 10:1,injector temperature:220℃ ,MS Detector.Results:16 components were identified and the total amount of fatty acids accounted for 94.19% of total Harp Seal Oil.The main components were myristic acid（4.55%）,palmitic acid（8.13%）,palmitoleic acid（17.55%）,oleic acid（25.75%）,eicosenoic （11.19%）, eicosapentaenoic acid（6.82%）,erucic（2.38%）, docosapentaenoic acid（4.16%）, docosahexaenoic acid（7.94%）.Conclusion:The method is reliable and can be applied to analyze the oil components from Harp Seal Oil.更多还原