【摘要】 目的　从自由基生物学和自由基医学的角度探讨糖尿病患者由于体内高血糖引起的氧化应激反应和脂质过氧化反应 ,导致肾脏等器官潜在的氧化损伤和脂质过氧化损伤后谷胱甘肽抗氧化系统变化情况。方法　采用随机对照设计 ,根据尿微量白蛋白定量将糖尿病患者分为糖尿病无肾病 (DNN)组和早期糖尿病肾病 (DEN)组两组 ,用分光光度分析法检测了 88例糖尿病患者(36例DNN和 5 2例DEN患者 )和 5 2例健康成人志愿者 (HAV)的血浆过氧化脂质 (PLPO)含量和血浆谷胱甘肽 -S -转移酶 (GST)活性及全血谷胱甘肽 (GSH)含量、红细胞过氧化脂质 (ELPO)含量、红细胞谷胱甘肽过氧化物酶 (GPX)活性。结果　与HAV组上述各生化参数的平均值比较 ,DNN组和DEN组的GST、GPX平均值皆显著降低 ,差别均具有显著性 (P <0 .0 5或 0 .0 0 1 ) ;而ELPO、PLPO平均值皆显著升高 ,差别均具有显著性 (P <0 .0 0 1 ) ;但DNN、DEN组与HAV组的GSH各平均值差别却没有显著性 (P >0 .0 5 ) ;与DNN组比较 ,DEN组的ELPO值显著升高 ,差别具有显著性 (P =0 .0 1 ) ;其余各生化参数平均值位于DNN组和DEN组之间 ,差别均无显著性 (P >0 .0 5 )。结论　在糖尿病期和DEN期 ,由于氧化应激反应和脂质过氧化反应 ,消耗部分谷胱甘肽抗氧化物质而使该防御系统活性降低。更多还原

【Abstract】 Objective To observe change of glutathione(GSH) anti-oxidant defense system in early diabetic nephropathy Methods 88 diabetic patients(36 DNN and 52 DEN) and 52 healthy volunteers (HAV) were enrolled in a random control study design and concentrations of lipid peroxidase (LPO) in plasma(PLPO) and LPO in erythrocytes (ELPO) and the whole blood glutathione, as well as activities of glutathione peroxidase (GPX) in erythrocytes and glutathione-S-transferase (GST) in plasma were determined with spectrophotometric assays. Results Compared with two diabetic groups and the HAV group, the average value of the activity of GST,GPX were significantly decreased (P<0.05 or 0.001),and those of concentrations of PLPO and ELPO were significantly increased (P<0.001),but the average value of the concentration of GSH was no significantly altered (P>0.05). Compared with the DNN group and the DEN group,the average value of concentrations of GSH,PLPO and those of the activity of GST,GPX were no significantly altered (P>0.05),but that of concentration ELPO in the early diabetic nephropathy was significantly increased (P<0.05). Conclusion Determining GSH anti-oxidant(GSH,GPX,GST) may clinically detect diabetic early renal injures.更多还原