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人工三倍体新桑品种嘉陵20号的AFLP分析
AFLP Analysis of the Newly Breed Artificial Triploid Mulberry
【摘要】 利用AFLP分子标记技术, 从DNA水平对人工三倍体新桑品种嘉陵20 号(2n=3x=42) 及其亲本以及与嘉陵20号同亲本的2 个三倍体(2n=3x=42) 桑树材料与10 个二倍体(2n=2x=28) 桑树材料进行了遗传背景分析。获得了它们的指纹图谱、遗传距离及遗传相似系数, 并绘制出了它们的UPGMA聚类图。研究结果发现: 嘉陵20 号的母本7920 (2x;♀) 与父本西庆四号(4x; ♂) 间的相似系数为0 6612。这为今后更具强大杂交优势和多倍体优势的新型人工三倍体新桑品种选育时杂交亲本的选择与组配提供了较有用的参考依据。
【Abstract】 In this study,genetic background of Jialing 20,their parents and Jialing 20 brothers are analyzed by the AFLP(Amplified Fragment Length Polymorphism),from which the genetic distance,similarity coefficient and UPGMA dendrogram of the materials are obtained.These will provide reliable evidence for the selection and tissue mating of parents.It is found the similarity coefficient between 7920 (2x;♀) and Xiqing 4 (4x;♂),which are parents of Jialing 20,is 0.6612.These will be helpful in the breeding of new artificial triploid mulberry with strong heterosis and multiploid superiority.
【Key words】 Mulberry; Artificial triploid; Genetic background; AFLP analysis;
- 【文献出处】 云南植物研究 ,Acta Botanica Yunnanica , 编辑部邮箱 ,2005年02期
- 【分类号】S888.3
- 【被引频次】7
- 【下载频次】84