【摘要】 在DMEM培养液中添加10%小牛血清(NBS)及10%二甲基亚砜(DMSO)作为冻存液,慢速降温冷冻,液氮保存7日龄小鼠生精小管及完整睾丸,37℃水浴复苏,0 25%胰蛋白酶消化成单细胞,台盼蓝染色测定细胞复苏率。结果:生精小管及完整睾丸冷冻复苏后细胞复苏率分别为87 3%及85 0%,两复苏率之间无显著差异,与生精小管单细胞对照组相比也无显著差异。冷冻损失率分别为10 1%及12 4%。结果表明,对于7日龄小鼠生精小管及完整睾丸,以10%DMSO为抗冻剂,慢速降温冷冻,液氮保存,37℃水浴复苏是一种适宜的冷冻保存方法。更多还原

【Abstract】 Seminiferous tubules and whole testes of 7-day-old mice were slowly frozen in cryopreservative solution composing of 10% dimethylsulphoxide,10% neoborn bull serum and 80% DMEM medium, stored in liquid nitrogen, thawed in a 37℃water bath,and the recoveries were measured by trypan blue exclusion staining after testicular cells dissociated by 0.25% trypsin. The recoveries of seminiferous tubules and whole testes of 7-day-old mice were 87.3% and 85.0%, respectively. There was no significant difference between the two recoveries, and also showed no significant differences compared with single cell control. The cell loss rates after freezing/thawing were 10.1% and 12.4%, respectively. The results demonstrate that a two-step slowly freezing procedure is a suitable procedure for cryopreservation of 7-day-old mouse seminiferous tubules and whole testes in 10% DMSO freezing solution.更多还原