【摘要】 目的探讨大鼠视网膜缺血再灌注损伤后热休克蛋白70(HSP70)、核蛋白因子-κB(NF-κB)表达和损伤神经细胞凋亡的关系。方法前房加压法制作大鼠视网膜缺血再灌注损伤模型,28只大鼠随机分为正常组和缺血再灌注组,后者又按照再灌注后不同时间段分为1、6、12、24、48、72h组。应用末端脱氧核酸转移酶介导的脱氧三磷酸尿苷缺口末端标记(TUNEL)法检测视网膜神经细胞凋亡指数(AI),过氧化物酶标记的链酶卵白素(SP)免疫组织化学法检测视网膜组织中HSP70、NF-κB的表达。结果视网膜神经细胞的凋亡出现于再灌注后6h,并逐渐递增,24h达到高峰,48h开始下降,72h不明显。HSP70在再灌注后1h即有表达,随时间段延长表达逐渐增加,至再灌注后24h达到高峰,之后渐下降。NF-κB的表达变化与凋亡细胞变化的规律基本一致。结论视网膜缺血再灌注损伤导致神经节细胞的凋亡;HSP70和NF-κB在视网膜缺血再灌注损伤中表达升高,对神经细胞的凋亡起着重要的调节作用。更多还原

【Abstract】 ObjectiveTo explore the relationship between the expression of HSP70,NF-κB and the cell apoptosis in retinal ischemia/reperfusion injury.MethodsThe animal model of retinal ischemia/reperfusion injury was created by transiently elevating introcular pressure (IOP).A total of 28 rats were divided into normal and ischemia/reperfusion groups,and the latter was subdivided into the 1st,6th,12nd,24th,48th and 72nd hours group after retinal reperfusion.Apoptosis was assessed by the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) method,and the expressions of HSP70 and NF-κB were studied by Streptavidin Peroxidase( SP ) immunohistochemistry.ResultsNo NF-κB positive cells,apoptosis cells and expression of HSP70 were observed in the retina of normal rats.There was a number of TUNEL positive cells in the 6th-24th hour after ischemia/reperfusion followed by a decrease at the 48th hour with the maximum at the 24th hour,and NF-κB protein expression followed a similar pattern with TUNEL detection.The expression of HSP70 was increased at the 1st hour after ishemia/reperfusion and reached its peak at the 24th hour,and decrease of expression level occurred at the 48th hour.ConclusionRetinal ischemia/reperfusion induces cell apoptosis in the retina.The expressions of HSP70 and NF-κB can regulate this procedure.更多还原