【摘要】 目的研究糖尿病大鼠白内障形成过程中晶状体上皮细胞(LEC)诱导型一氧化氮合酶(iNOS)mRNA表达的变化及生化改变。方法用链脲佐菌素(STZ)45mg/kg腹腔注射复制糖尿病大鼠白内障动物模型后分为对照组、STZ组和治疗组(应用葛根素腹腔注射)。分别于实验第20、40、60d时取材,采用逆转录聚合酶链反应(RTPCR)技术及生化方法观察糖尿病大鼠LECiNOSmRNA表达的变化及其对一氧化氮(NO)和一氧化氮合酶(NOS)生成的影响。结果糖尿病大鼠白内障形成过程中iNOSmRNA表达变化并伴有NO和NOS生成增加。对应用葛根素的糖尿病大鼠观察表明,实验后期iNOSmRNA的表达明显减弱,伴有NO和NOS活性明显下降。结论iNOS诱生并大量增加是过氧亚硝基阴离子(ONOO-)在糖尿病大鼠LEC中产生的重要基础。ONOO-参与糖尿病大鼠晶状体氧化而发生白内障。葛根素通过对抗ONOO-对糖尿病大鼠LEC的氧化作用而间接调控iNOS基因的表达。更多还原

【Abstract】 ObjectiveTo study the expression of inducible nitric oxide synthase mRNA (iNOS mRNA) and biochemical changes during the formation of diabetic rat cataract.MethodsSeventy-two rabbits received intraperitoneal injection of streptozotocin [STZ,45mg/(kg·w)] to establish an animal model of diabetic cataract. They were then assigned to treated group (peritoneal injection of puerarin) and STZ group (36 rabbits for each group),and the remaining 36 normal rabbits served as control group.Morphologic changes of lens were observed with slit lamp biomicroscope.Samples of lens were collected at the 20th , 40th and 60th day.The changes of iNOS mRNA in lens epithelium cells(LEC),production of NO and nitric oxide synthase (NOS)were determined with reverse transcription-polymerase chain reaction(RT-PCR) and biochemical method,respectively.ResultsExpression of iNOS mRNA in the LEC,increasing of NO and NOS were observed during the formation of rat diabetic cataract,and distinctly faint expression of iNOS mRNA and decreasing of NO along with NOS were seen in the treated group.ConclusionInduction and increasing of iNOS are an important basis of production of ONOO - in the LECs in diabetic rat.Purerin can regulate the expression of iNOS mRNA indirectly by antagonizing the oxidation of ONOO - in LECs.更多还原