【摘要】 目的　探讨单核细胞趋化蛋白 1 (MCP 1 )在早期糖尿病大鼠视网膜中的表达及其与糖尿病视网膜病变(DR)的关系。 方法　Wistar大鼠随机分为正常对照组 (NC组 )和糖尿病模型组 (DM组 ),每组 12只。大鼠腹腔注射链脲佐菌素(STZ)诱发糖尿病模型。8周时全部处死,采用Western免疫印迹法和免疫组织化学法,分析MCP 1在早期DM大鼠视网膜中的表达,对免疫印迹结果采用计算机图像分析系统处理。 结果　8周时,免疫印迹结果显示视网膜中MCP 1的表达DM组明显高于NC组(P<0 01),免疫组织化学法显示 8周时DM大鼠视网膜表面血管、内核层均可见MCP 1阳性着染细胞。 结论　MCP 1在视网膜组织中的表达与DR的发生有密切关系。更多还原

【Abstract】 Objective To investigate the expression of monocyte chemoattractant protein 1(MCP 1) in retina of early diabetic rats and its correlation with diabetic retinopathy(DR). Methods Twenty four healthy adult male Wistar rats were randomly divided into normal control(NC) group and diabetes mellitus(DM) group.DM of rats was induced by injection of streptozotocin(STZ) intraperitondally.MCP 1 expression in retina was assessed by Western blot analysis and immunohistochemical method in the 8th post injected week.The result of Western bolt was analyzed by computer picture analysis system. Results The computer picture analysis indicated a significant difference in the expression of MCP 1 between the DM group and the NC group ( P <0 01).MCP 1 was expressed in retinal vessel and inner nuclear layer in diabetic rats by immunohistochemical method. Conclusion The expression of MCP 1 in the retina may have a close correlation with the development of DR.更多还原