【摘要】 目的:采用高速逆流色谱技术分离提纯浙贝母中的活性成分贝母素甲,贝母素乙。方法:采用氯仿-无水乙醇-0.2mol·L-1盐酸(4∶3∶2)系统,上相为固定相,下相为流动相。主机转速为800r·min-1,流动相流速为1.2mL·min-1。结果:一次进样分离得到贝母素甲、贝母素乙,经高效液相(HPLC)分析纯度,经MS,1HNMR鉴定结构。结论:利用本实验条件可从浙贝母中分离制备贝母素甲,贝母素乙,两者纯度分别达到:97.5%,94.5%。更多还原

【Abstract】 Object: High Speed Countercurrent chromatography was applied to separation and purification of peimine and peiminine from Bulbus Fritillariae Thunbergi. Method: The solvent system used for HSCCC was chloroform- ethanol-0.2mol·L-1 hydrochloric acid(V/V, 4∶3∶2),the organic layer was used as stationary phase,the aqueous phase was used as mobile phase .The rotational speed of main engine was 800 r·min-1, the flow of mobile phase was 1.2 mL·min-1. Result: The purified peimine and peiminine were dentified and analyzed by HPLC,1HNRM,MS. Coclusion: Under this condition peimine and peiminine can be separated and purified from Bulbus Fritillariae Thunbergi. purities were respectively 97.5% and 94.5%.更多还原